#471 You are required to investigate the effects
You are required to investigate the effects - Biology
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As a recent graduate of RMIT University you have just been employed (3-year contract) by a world-renowned infectious diseases institute located in Victoria, Australia. You are required to investigate the effects of the novel small molecule anti-viral (DAD030981) to determine the global metabolic changes which take place in the tissue of lungs of monkeys infected by SARS- CoV-2 (Infected Group) vs the tissue of lungs of healthy monkeys (Control Group). You have been asked by your laboratory head to design and carry out a “metabolomics experiment.” Q1. What experimental design would you propose to your lab head? He will first need to approve this and then discuss with you how you are going to prepare samples and analyse them before you begin. What kind of metabolomics approach will you propose to your lab head and why? What considerations with respect to your experimental design should you propose?
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Case control experiment wiould be proposed to the lab head. Case control experiment will be used to assess the potential risk related to anti-viral (DAD030981) in the tissue of lungs of monkeys infected by SARS-CoV-2 (Infected group) vs the tissue of lungs of healthy monkeys (Control Group).
Proposed metabolic approach
Both groups of the subjects will be kept separately to prevent the cross infection first. The number individuals in each groups will be according to the requirement of statistical method.
Dose of the anti-viral (DAD030981) drug will be decided first for both groups according to the assessment of toxicity and efficacy.
I will go for statistical analysis to determine the actual effect of anti-viral (DAD030981) drug. The significant and non-significant effect of the anti-viral (DAD030981) drug will be analysed by testing the null hypothesis through analysis of variance method.
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