#463 As a recent graduate of RMIT University

As a recent graduate of RMIT University - Biology

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As a recent graduate of RMIT University you have just been employed (3-year contract) by a world-renowned infectious diseases institute located in Victoria, Australia. You are required to investigate the effects of the novel small molecule anti-viral (DAD030981) to determine the global metabolic changes which take place in the tissue of lungs of monkeys infected by SARSCoV-2 (Infected Group) vs the tissue of lungs of healthy monkeys (Control Group). You have been asked by your laboratory head to design and carry out a “metabolomics experiment.”

Q3. Your lab head has now asked you to analyse the polar metabolite extract on the laboratory’s GC-MS.

Briefly describe how it may be possible to analyse polar metabolites on the GC-MS.

What additional sample should you run with you experiment and why?

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Free Chegg Answer

GC-MS analysis is a highly sensitive technique used to identify target and non target metabolomics of complex biological samples that play crucial roles in cellular Dynamics. GC can analysis even trace amount of non polar, polar and volatile compounds. There are limitations to analyse polar compounds due to their low volatile nature and thermoliability. In the presalting target metabolomics, the polar compounds will also be eluted alone with the salt. To prevent this, the amine,the thiol, or hydroxyl groups of polar metabolites are derivatized with trimethylsilyl (TMS) derivatives and tert-butyldimethylsilyl (TBDMS) which would increase the stability and volatility of metabolites.